Yvonne Stahl holds a diploma degree in Biology from the University of Cologne (Germany). She finished her PhD under the supervision of Prof. Peter C. Morris at Heriot-Watt University, Edinburgh (UK) in 2003. Thereafter, she worked as a Research Fellow and then as a group leader at the Institute for Developmental Genetics, Heinrich Heine University, Düsseldorf (Germany). She habilitated in...
Carsten Grashoff studied Applied Science at the University of Freiberg in Germany and performed his diploma studies at the Robert-Koch Institute in Berlin. He worked as a PhD student at the Max Planck Institute of Biochemistry in Munich and obtained his PhD degree in 2007, before moving for his postdoctoral work to the University of Virginia (USA). In 2010, he was awarded an Emmy-Noether...
The integration of contemporary genetic and optical microscopy tools is permitting researchers to control and observe biochemical networks in single living cells and organisms. I will provide a brief introduction and examples on how we can use FRET, FLIM and optogenetics to study how cell biochemistry encodes for cellular decisions. I’ll discuss some of the challenges and opportunities that...
Sumeet Rohilla studied physics (M.Sc.) and optics (M.Tech) at the Indian Institute of Technology Delhi, India.
He worked as a Marie Curie Research Fellow (Ph.D.) at the Charité – Universitätsmedizin Berlin and PicoQuant GmbH, where the primary focus of his research work was to develop spectrally-resolved fluorescence lifetime imaging (spectral-FLIM) systems and corresponding data analysis...
Werner Zuschratter studied Biology at TU-Darmstadt and managed the Special Laboratory for Electron- and Laserscanning Microscopy at Leibniz Institute for Neurobiology (LIN) for more than 25 years before he became coordinator and head of the Combinatorial NeuroImaging Core Facility at LIN (CNI). Starting his career in the field of electron microscopy his research activities focus on...
Gerhard Holst, Head of Research & Science Department, PCO AG, Kelheim, Germany
Gerhard Holst graduated at the Technical University Aachen, Germany, with a Diploma in Electrical Engineering in 1991 (Information Technology) and went on to complete his Doctorate at the University of Dortmund in collaboration with the Max-Planck-Institute for Systems Physiology in Dortmund, Germany from 1991 -...
Dr. Periasamy received his Ph.D. at the Indian Institute of Technology, Madras and postdoctoral training at the University of Washington Seattle. Currently, Dr. Periasamy is a prof. of Biology and Biomedical Engineering, the director and founder of the internationally known WM Keck Center for Cellular Imaging (KCCI), University of Virginia, Charlottesville. Dr. Periasamy is one of the...
Rupsa Datta is an Assistant Scientist in the laboratory of Dr. Melissa Skala, a Professor of Biomedical Engineering at the University of Wisconsin – Madison and lead investigator at the Morgridge Institute for Research, Madison. Rupsa graduated from University of California, Irvine with Ph.D in Biomedical Engineering. Trained in fluorescence lifetime imaging microscopy, her research entails...
Angelika Rueck studied chemistry at the University of Ulm and did her PhD in physical
chemistry. For more than 20 years she led the research on photodynamic therapy and
advanced microscopy at the Institute for advanced Photonics and Optics (ILM) in Ulm. Since
7 years she held the position of the director of the core facility and bioimaging research group
confocal and multiphoton microscopy...
In many biological research projects, a high spatial and temporal resolution for the observation of in vivo protein interaction is needed, e.g. in order to follow changes of interactions and complex formation over time. In vivo Fluorescence or Förster resonance energy transfer (FRET) measurement allows detailed analysis of interacting molecules in their natural environment at a subcellular...
The fluorescence lifetime of many fluorophores is highly dependent on their environment, making fluorescence lifetime imaging (FLIM) a sensitive tool for analyzing subcellular distributions of certain ions or gradients in viscosity or hydrophobicity. In this workshop, we will exemplary demonstrate the usage of a pH-sensitive dye to monitor changes in lysosomal pH (Tannert et al, submitted)....
The workshop’s aim is to introduce another potential use of lifetime: dye separation. Much like absorption and emission spectra, the fluorescence lifetime depends on the intrinsic characteristics of each fluorescent dye and its surroundings. If two fluorochromes have the same emission properties, separation by color may be impossible. But their lifetime can still be quite different. This...
Fluorescence lifetime provides a new perspective for STED nanoscopy. This workshop will focus on Tau-STED, a new concept for STED Nanoscopy that exploits the fluorescence lifetime gradient induced by the STED beam in a novel way to identify and remove uncorrelated background, improve image quality and increase image resolution in an automated way. This is only possible thanks to the unique...
Description
During this session, we explore the potentials of autofluorescence imaging to investigate the cellular metabolic state.
Adenosine triphosphate (ATP), the energy molecule of the cells is the product of different energy production pathways - glycolysis and Krebs cycle and oxidative phosphorylation. Two co-factors plays important role in the energy production pathways: NAD(P)H...
This workshop will show the analysis capabilites of the current Leica LAS X software release,focusing on the integrated Phasor Plot analysis.
